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KMID : 0525720190240030179
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Journal of Chitin and Chitosan
2019 Volume.24 No. 3 p.179 ~ p.185
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Screening of Cellulolytic Microorganism and Characterization of Cellulase from Bacillus subtilis PFB-1
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Kim Young-Su
Yu Sang-Mi
Park Chang-Su
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Abstract
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Six different kinds of strain were isolated from environmental soils in Kyeongsans-si, Kyeonbuk, Korea as cellulolytic microorganism due to their ability of enzyme activity for carboxymethylcellulose (CM-cellulose). Based on the analysis of 16s rRNA gene, they are identified with 3 kinds of Bacillus subtilis, 2 kind of Bacillus sp. and one kind of Bacillus amyloliquefaciens.Among these strains, one strain was selected from its high cellulase activity and named Bacillus subtilis PFB-1 because of 99% of identity with Bacillus subtilis ZFJ-8 (GenBank EU931563). B. subtilis PFB-1 showed the maximum cellulase activity after 24 h cultivation and the cellulase from B. subtilis PFB-1 exhibited the highest cellulase activities at the conditions of pH 5.0 and 50°C, for CM-cellulose. The cellulase from B. subtilis PFB-1 was stable at pH 4.0~6.0 range and until 40°C for 30 min perfectly. In the substrate specificity of the cellulase from B. subtilis PFB-1 for the various cellulosic substrates, the enzyme had the highest activity for CM-cellulose. In the hydrolysis of CM-cellulose by cellulase from B. subtilis PFB-1, the optimum conditions for substrate concentration and enzyme activity were investigated 1.5% and 3.0 U/ml, respectively.
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KEYWORD
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Bacillus subtilis, Cellulase, Characterization, Environmental soil, Screening
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